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E. coli ORFmers
E. coli ORFmers are PCR primer pairs that have been designed to amplify
each open reading frame (ORF) in E. coli, from the start codon to the
stop codon. Genosys Biotechnologies, Inc. offers 4,290 primer pairs,
representing all protein-coding genes. The E. coli ORFmers are the
direct result of a collaboration with the E. coli Genome Group, headed
by Dr. Fred Blattner at the Department of Genetics, University of
Wisconsin-Madison (http://www.genetics.wisc.edu/). The PCR primer pairs
were designed with the following features:
All upstream or "amino-terminal" primers contain an
"ATG"
start codon (A-primer).
All downstream or "carboxyl-terminal" primers contain a
"TAA" stop codon (C-primer).
A Sap I restriction site has been incorporated into a 5'
"adaptamer" sequence in all primers. The "adaptamer" sequence is 13
bases in length. The Sap I enzyme cleaves outside its recognition
sequence,
generating a PCR product with an 5'-ATG-3' overhang at one end and a
5'-TTA-3' overhang at the other end. This feature has been included to
facilitate cloning into a vector with the corresponding sites.
A pair of "Universal Adaptamer" PCR primers are
available to
allow re-amplification of any ORF PCR product.
ORFmer Primers are sorted according to gene "Functional
Group". Primers from any functional group may be ordered as individual
tubes
or in a 96-well micro-plate format. There are a total of 23
functional groups.1
E. coli ORFmer Pricing:
$20 per PCR primer pair for orders of 1-20 pairs (2,000
pmol
of A- and C-primers in individual tubes)
$12 per PCR primer pair for orders of 21 or more pairs
(2,000 pmol of A- and C-primers in individual tubes).
$850 per 96-well plate (2,000 pmol of A- and C-primers
combined per well {4000 pmol total}, 96 primer pairs).
Please contact your account representative if you are
interested in purchasing a complete set of primers in tube or
micro-plate
formats (45 micro-plates, 4,290 PCR primer pairs).
1. Blattner, F.R., Plunkett, G., Bloch, C.A., Perna, N.T.,
Burland, V., Riley, M., Collado-Vides, J., Glasner, J.D., Rode, C.K.,
Mayhew, G.F.,
Gregor, J., Davis, N.W., Kirkpatrick, H.A., Goeden, M.A., Rose, D.J.,
Mau, B. and Shao, Y. The complete genome sequence of Escherichia coli
K-12. Science 277:1453-1462 (1997).
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