W A R N I N G S   A F F I X E D   T O L A B O R A T O R Y   D O O R S A T   T H E   M A S S A C H U S E T T S I N S T I T U T E   O F   T E C H N O L O G Y . - - - - [As a service to our readers, we will be gathering and reprinting notices posted on laboratory doors at the Massachusetts Institute of Technology. The following notice was found attached to a refrigeration unit outside an MIT chemistry laboratory. It appears below unaltered. (Note: if you have notices that you have found posted to doors at MIT, please email them to mcsweeneysmail@yahoo.com with "MIT Doors" in the Subject header.)] - - - - SAMPLES LIST -- Ming Chen I: One box in the -20 freezer: [all of the samples are dried down] **MIB purified, aka peak 2 [see HPLC trace / MS data] (several eppendorfs, sample vials) Sequence: Lys(biotin)-Tyr-His-Amb-Leu-Thr-Val-Thr-Nph-NH2 - crude MIB -- cleaved off resin, not run on HPLC (several epps) - oxidized MIB, aka peak 1 [see HPLC trace / MS data] (sample vial) --> can probably be recovered to MIB by reducing biotin sulfur. - MI peptide -- control, non-biotinylated peptide (eppendorf) Sequence: Lys(acetyl)-Tyr-His-Amb-Leu-Thr-Val-Thr-Nph-NH2 - VT inhibitor in DMSO (100 µM) Two ON RESIN samples: 1) Boc-Lys(Fmoc)-Tyr(tBut)-His(tBut)-Amb(Boc)-Leu-Thr(tBut)-Val-Thr(tBut)-Nph-NH2 - complete peptide sequence, but not biotinylated - can be used to introduce other labels, not biotin 2) Fmoc-His(tBut)-Amb(Boc)-Leu-Thr(tBut)-Val-Thr(tBut)-Nph-NH2 - before manual synthesis step (7/9/98) - NOTE: before addition of tyrosine residue (which was supposed to increase the efficiency of cross-linking to aromatic residue) - can be used to make MIB without tyrosine.

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